Oil Recovery Test Using Bio surfactant of Halo tolerant Bacteria Brevundimonas diminuta and Bhurkholderia glumae at variation of NaCl Salt Concentrations

BAMBANG YUDONO, MUHAMMAD SAID, SRI PERTIWI ESTUNINGSIH, AULIA KARIMA

Abstract


Oil recovery test has been done by using crude biosurfactant from Brevundimonas diminuta and Bhurkholderia glumae indigenous halo tolerant bacteria with the vatiation of NaCl salt concentration 0; 1.5; 3; 4.5; 6; and 7.5%. Oil recovery test was obtained by determining % TPH (Total Petrolem Hidrocarbon). The sample concentration was 28.19% TPH, it was extracted by using biosurfactant of  Brevundimonas diminuta and Bhurkholderia glumae bacteria, the optimal salitnity conditions were  at 3, 4.5% salt concentrations with the value oil recovery as much as 50.41, 69.97 % respectively. Oil components which extraction by biosurfactant were analyzed by using GC-MS (Gas Chromatography-Mass Spectrophotometry). The result from analyzes GC-MS could be concluded that bacteria Brevundimonas diminuta could dissolve hydrocarbon compounds short chain carbon atom at fraction <C10–C14 and long chain carbon atom at fraction >C22. <C10, C11-C14dan C15-C17  and Bhurkholderia glumae could dissolve hydrocarbon compounds short chain carbon atom at fraction <C10–C14 and long chain carbon atom at fraction >C22 according to the retention time.

Keywords


recovery oil, ;crude biosurfactant; indigenous halo tolerant bacteria; Brevundimonas diminuta; Bhurkholderia glumae

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DOI: http://dx.doi.org/10.5454/mi.11.3.%25p

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